What are the dilution methods for antibodies? -Huaqiang Electronic Network

Our company combines the habit of [antibody] to specifically bind to the corresponding antigen. Different classes of immunoglobulins can bind different types of cells, produce different sputum, and participate in immune responses. The antibodies sold by our company are highly specific, and the purification method of affinity-purified proteins makes the experimental results more accurate. Strict control of product quality, a variety of dilution methods to help you adjust the antibody concentration. It can meet the special requirements of product types, packaging and purity of R&D customers, and also meet the comprehensive needs of production enterprises from small test, pilot test to large-scale production. Dilution method: Method 1. Before the experiment, dilute the lyophilized powder antibody with sterile distilled water (or diluted with PBS), and dispense the diluted antibody into 5-10 (20ulx5 or 10ulx10) and store at -20 °C. Use one to take one to avoid repeated freezing. Method 2. Before the experiment, the lyophilized powder antibody can also be diluted with sterile distilled water (50 ul (or 50 ul diluted in PBS) into a stock solution: add 50% glycerol, and store at -20 ° C, how much to use. Method 3. Pre-test can do several gradients: 1:100, 200, 400 background height can also be done, choose an optimal dilution ratio, and then officially, the best results. Dilution of working fluid - use antibody dilution. Comparison of paraffin sections and frozen sections: (1) It is not necessary to make paraffin sections for frozen sections. This is the conclusion I asked from a teacher today. Because high temperature baking of the paraffin section may destroy the antigenicity of the tissue. If the antigenicity of the tissue is stable, it can be used for paraffin sectioning; however, if it is required to be paraffin-sliced, it can be used for frozen sectioning. (2) The advantage of frozen section is that it can better preserve the antigen immunological activity of the tissue, and does not require antigen retrieval in immunohistochemistry. The disadvantage is that the cells are easy to form ice crystals and destroy the cell structure, which may cause the antigen to diffuse; the thickness of the slice is thicker than that of the paraffin, and the film is not as beautiful as paraffin. When you buy a primary antibody, the catalogue says what kind of slice to make. If it says that it can only be frozen, you can't make paraffin. If you write both, you can do it. (3) The advantages of paraffin section can maintain the morphological structure of tissue cells, and easy to store at room temperature, while frozen sections are more troublesome, must exist in a low-temperature refrigerator of -80 degrees, especially for in situ hybridization. In order to prevent RNA degradation, preservation has always been important. Since the paraffin section can be cut to about 4 microns, the in situ hybridization probe is easy to penetrate into the tissue, is easy to succeed, and the color/morphology obtained is better than that of the frozen section. 【【Products Recommended】】 bs-15496R ALDH3A1 aldehyde dehydrogenase 3 family, member A1 antibody bs-12963R phospho-alphaBCrystallin (Ser59) phosphorylation of heat shock protein β5 / αb crystal protein / α- crystallin b chain antibody bs-12368R ACTG2γ2 actin antibody bs -12392R AESgp130 GAM antibody binding protein bs-12414R AMHR2 Mullerian hormone 2 receptor antibody bs-12415R ACTR1 1A activin receptor antibody bs-12416R ACTR2 2A activin receptor antibody bs-12417R ActivinReceptorTypeIIB activin receptor antibody bs 2B -15163R No. C2orf882 chromosome 88 open reading frame bs-7144R ARHGEF18G antibody protein coupled receptor antibody ARHGEF18 bs-3763R ActivinAReceptorTypeIB 1B activin receptor antibody bs-3751R ARA24 androgen receptor associated protein 24 antibody bs-0347R AmyloidPrecursorProtein amyloid peptide precursor protein antibody (C-terminus) bs-3920R ADCY2 2 adenylate cyclase antibodies bs-5037R APOC3 apolipoprotein C3 antibody bs-5038R ApolipoproteinE4 apolipoprotein E4 antibody bs-3830R AMBRA1 autophagy-related genes AMBRA1 antibody bs- 5200R Phospho-ATG4C (Ser177) phosphorylation autophagy-associated protein 4C antibody bs-5205R Phospho-ATG4C (Ser398) phosphorylation autophagy-associated protein 4C antibody bs-3007R phos pho-ASK1 (Ser966) phosphorylated apoptosis signal-regulated kinase 1 antibody bs-3008R phospho-ATXN1 (Ser775) phosphorylation dysfunction protein 1 antibody bs-2732R ATXN1/Ataxin-1 spinal cerebellar dysfunction protein 1 antibody bs-3028R phospho-Amyloid PrecursorProtein (Thr668) phosphorylated APP amyloid peptide precursor protein antibody bs-3029R phospho-ASK1 (Ser967) phosphorylation apoptosis signal-regulated kinase 1 antibody bs-3031R phospho-ASK1 (Thr845) phosphorylation apoptosis signal Regulatory kinase 1 antibody bs-3033R phospho-ATF2 (Thr69/71) phosphorylation-activated replication factor 2 antibody bs-3034R Phospho-ATP1A1 (Tyr10) phosphorylated sodium potassium ATPase protein a1 antibody bs-3035R Phospho-ATP1A1 (Ser16) phosphoric acid Sodium potassium ATPase protein a1 antibody bs-2745R AcetylCoACarboxylase acetyl-CoA carboxylase antibody bs-3036R Phospho-AcetylCoenzyme Acarboxylasealpha (Ser78) phosphorylated acetyl-CoA carboxylase antibody bs-3038R Phospho-Ack1 (Tyr859+860) phosphorylation Ack1 antibody bs-3025R phospho-AMPKalpha-1 (Ser485+Ser491) phosphorylated adenosine monophosphate-activated protein kinase alpha1 antibody bs-3026R phospho-AMPKbeta1 (Ser108) phosphorylated adenosine monophosphate-activated protein kinase beta1 antibody bs-30 22R phospho-ALK (Tyr1604) phosphorylation anabolic lymphoma kinase antibody bs-2272R phospho-ATM (Ser1981) phosphorylation telangiectasia ataxia mutein antibody bs-3037R phospho-ATPcitratelyase (Ser455) phosphorylated triphosphate Adenosine citrate lyase antibody bs-3030R phospho-ATR (Ser428) phosphorylation ATR antibody bs-7960R AMN1 nuclear relocalization and spindle checkpoint protein AMN1 antibody bs-7957R ALG11 asparagine linked glycosylation 11 antibody bs-3777R ASF1A cell senescence-associated protein antibody ASF1A aminoglycoside phosphate bs-8457R AGPHD1 domain antibody bs-4119R ASGPR1 asialoglycoprotein receptor antibody bs-9100R ARSF aryl sulfatase F antibody bs-6980R AKAP5A kinase anchoring protein 5 antibody bs-4807R ATXN3L spinocerebellar ataxia 3 protein antibody bs-9297R ASTE1ASTE1 antibody bs-5212R phospho-APG4B (Ser309) phosphorylation autophagy-related protein antibody bs-9790R AFAP 4B actin-related protein 1 antibody bs-4235R ADORA1 adenosine A1A receptor antibody bs-4231R ARSB arylsulfatase B antibody bs-4234R ABATγ aminobutyric acid transaminase antibody bs-6513R A2BP1 ataxia protein 2 binding protein 1 antibody bs-4236 R ADAM17 tumor necrosis factor alpha converting enzyme